Integrative analysis of chloroplast genome, chemicals, and illustrations in Bencao literature provides insights into the medicinal value of Peucedanum huangshanense

The genus Peucedanum L. (Apiaceae) is a large group comprising more than 120 species distributed worldwide. Many plants of the have been studied and used in traditional Chinese medicine. In 2020, new species, huangshanense Lu Q. Huang, H. S. Peng & Chu, was found Huangshan Mountains Anhui Province, China. However, little known about its medicinal properties. Thus, objective this study to explore potential value P . relationship with other species. Through textual research on illustrations Qianhu Bencao literature, it can be inferred that at least five Therefore, we chose these together for subsequent research. We conducted morphological, chloroplast genome, chemical analyses six including newly discovered P. genomes showed typical tetrad structure, gene structure content were similar conservative. There significant differences genome size expansion inverted repeat boundary. nucleotide polymorphism analysis, screened 14 hotspot mutation regions as specific molecular markers taxonomy Our results an inversion trnD-trnY-trnE which developed marker identification. Phylogenetic analysis phylogenetic trees had high support resolution, strongly supports view not monophyletic group. closest genetic ampliatum K. T. Fu, followed by harry-smithii Fedde ex Wolff. Furthermore, main coumarins most those japonicum Thunb. summary, our lays foundation systematic classification sheds light